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Image Search Results
Journal: bioRxiv
Article Title: Genome and epigenome engineering CRISPR toolkit for probing in vivo cis -regulatory interactions in the chicken embryo
doi: 10.1101/135525
Figure Lengend Snippet: (A) Schematic of dCas9-VP64-MCP SAM complex. (B) One sgRNA targeting the Sox10 promoter co-electroporated with dCas9-VP64 and MCP-VP64 causes a premature activation of Sox10 transcription on the experimental (left, magenta) as compared to the control side (right, blue), 50% embryos, n=12, *p<0.05 ). Results confirmed in three independent experiments; representative embryos are shown, error bars represent the standard deviation.
Article Snippet: The MCP-VP64 construct was generated by removing the IRES-H2B-RFP cassette from pCI_H2B-RFP vector ( ) and inserting the MCP and
Techniques: Activation Assay, Control, Standard Deviation